How Can I Generate a Hotspot Matrix?

The Hotspot Matrix is a useful predictive tool that maps how much each fluorochrome contributes spread into others. High-value cells are called 'hotspots' and highlight problematic dye pairings likely to cause spectral overlap, unmixing-dependent spread (UDS), and reduced resolution, especially for low-expression markers.

The Hotspot Matrix is generated in the Make Unmixing Matrix task in OMIQ. To learn more on how to use the Make Unmixing Matrix task, please see our article Create and Apply a Spectral Unmixing Matrix.