Validate the quality of the actual spectral signatures of single stained controls to the expected spectral signatures with the OMIQ-EasyPanel integration. This feature is available to all OMIQ users. You can readmore about the full functionality of EasyPanel, an intelligent and automated flow cytometry panel designer here: https://www.easypanel.ai.
1. Create an Unmixing Matrix
Create an unmixing matrix by following the instructions in our support article Create and Apply a Spectral Unmixing Matrix.
2. Validate with EasyPanel
2.1 In the Make Unmixing Matrix Task
You can validate the spectral signatures in the Make Unmixing Matrix Task.
Once you have generated your unmixing matrix, click on Validate with EasyPanel. This will open a new tab in the EasyPanel website that shows the Actual vs Expected Similarity of your single stained controls.
Actual vs Expected Similarity Score
Score = 1: Actual spectral signature is the same as the Expected spectral signature.
Score < 1: Indicates a difference between the Actual spectral signature and the Expected spectral signature.
The image above shows the web page in EasyPanel. This shows the Actual vs Expected Similarity as a score and as a spectral signature graph of your single stained controls. The example highlights BUV395 with a similarity score of 0.98.
The example above highlights DAPI with a similarity score of 0.7. Some causes of a low similarity score are contamination of the single stained controls, wrong single stained controls, or dye/fluorophore degradation due to poor storage or exposure to light.
2.2 In the Unmixing Task
You can also validate the spectral signatures within the Unmixing Task.
After you have pasted the unmixing matrix, click on Validate with EasyPanel. This will open a new tab in the EasyPanel website that shows the Actual vs Expected Similarity of your single stained controls.