Backgating is designed to help a user explore their gating strategy. It shows the final gated population within the population of it's ancestors. It is intended to help you visualize your population of interest within the distribution of the main population. This can lead you to decide whether your gates are produced correctly. You can view the Backgating Hierarchy as subplots in OMIQ's Gating task. You can adjust the gates in the subplots. This will update the statistics and provide feedback on how this adjustment affect your gated events in downstream plots.
You can easily create and export the Backgating Hierarchy in a Figure as well. To learn more about this, please see our article View Your Backgating Hierarchy in Figure.
1. Add a Gating Task
Click Add new child task and select Gating from the task selector.
2. Build your Gating Tree
Build your gating tree in the Gating Task. To learn more, visit our support article Performing Manual Gating in OMIQ.
3. View the Backgating Hierarchy
Select the gate that you would want to display the backgating hierarchy for.
The selected gate is indicated by the green circles highlighting the points of the gate.
Click on See backgating.
The backgating hierarchy is then displayed as subplots under the main plotting area. Clicking the See backgating button again collapses the backgating plot visualization.
Moving the gates in the subplots will update the gating tree population and stats. Please note that you can only add or delete gates on the main plotting area and not the subplots.
You can add multiple plots in the gating task. To learn more about this, please see our article on Viewing Multiple Gating Plots in a Single Gating Task.