You can view multiple gating plots in a single gating task in OMIQ, allowing you to visually compare different files/groups in one gating task.
1. Add a Gating Task
Click Add new child task and select Gating from the task selector.
2. Build your Gating Tree
Build your gating tree in the Gating Task. To learn more, visit our support article Performing Manual Gating in OMIQ.
3. Adding Plots
3.1 Adding a Single Plot
Click on Add Plot(s). Select Single Plot.
The view on the selected plot (indicated by the green box around the plot) will be duplicated.
The image above shows a single plot added to the task. This will be a duplicate of the selected plot. This will include the file, filter, and the open subplots, if applicable. Here, the See hierarchy subplots were open in the original selected plot, therefore these subplots are also copied over.
The hierarchy subplots are not needed to add multiple plots to your gating task. To learn how to See hierarchy subplots, please see our article View the Gating Hierarchy while Gating.
3.2 Adding Multiple Plots
Click on Add plot(s). Select Multiple plots...
Select the files you would want to add plots for. Click Submit.
The plots are added horizontally.The view on the selected plot (indicated by the green box around the plot) will be applied to the added plots.
The image above shows multiple plots added to the gating task. The added plots will have the settings of the selected plot applied to them. This will include the filter and open subplots, if applicable. Here, the See backgating subplots were open in the original selected plot, therefore these subplots are also copied over.
The backgating subplots are not needed to add multiple plots to your gating task. To learn how to See backgating subplots, please see our article View the Backgating while Gating.
4. Working with Multiple Plots
Some actions can be performed on all open plots while some actions are restricted to a selected plot. Find out more information below.
New gates are made on a single plot. Though, by default, these are applied to all files and therefore all plots.
Drawing a gate will automatically collapse the subplots open on the plot that you drew the gate on.
The image above shows a new CD4-CD8- gate being created in Sample 1 Drug B.fcs and automatically applying the gate to other files, example given on Sample 2 Drug A (red arrow).
By default, gates in OMIQ are applied globally (all files). However, you can apply a Per File Mode or a Per Metadata Mode. To learn more about this, please see our Performing Manual Gating in OMIQ.
You can view See backgating Subplot(s) in one plot while viewing See hierarchy Subplot(s) in another.
The image above shows two different files open in the multiple plots view. The left plot is Sample 1 Media.fcs and the right plot is Sample 1 Drug B.fcs. Both plots have the backgating subplots open for the CD4 gate.
The selected gate is indicated by the green circles highlighting the points of the gate.
The image above shows the same files open in the multiple plots view. The left plot is Sample 1 Media.fcs with backgating subplots open for CD4 gate. The right plot is Sample 1 Media.fcs with hierarchy subplots open for CD4 gate.
You can have multiple plots open for the same file with different subplots open for different gates/filters. Equally, you can have multiple plots open for different files with the same subplots open for the same gates.
Deleting a gate will cause all the subplots open for that gate to automatically close regardless of whether the plots are selected.
To learn more about the different modes of deleting a gate, please see our article on Performing Manual Gating in OMIQ.
The top image above shows the CD4-CD8- gate selected in Sample 2 Drug B.fcs file plot (indicated by red arrow). Once the gate is deleted note that all the subplots open for that particular gate have automatically closed (bottom image).
There may be instances wherein you would want to select all the plots to change their Plot Settings (see Section 4.5), for example reducing the plot size for easier viewing.
Right click on a plot and click on Select all plot(s).
This will select all open plots as indicated by indicated by the green box around the plots.
You can use CTRL+A (Windows) or CMD+A (Mac) to select all plots. If you only wanted to select specific plots, CTRL+Click (Windows) or CMD+Click (Mac) to select multiple non-adjacent plots. Shift+Click (Windows and Mac) allows you to select a range of adjacent files.
To deselect the selected plots, click on a plot. This will select that plot.
Pressing Escape on your keyboard will deselect all plots and will automatically select the first plot (left-most plot) in the gating task.
You can modify the Plot Settings for multiple selected plots (see Section 4.4).
4.5.1 Bulk Change the Plot Resolution
Select the plots you would want to change the resolution for.
In the Plot Settings look for Resolution and change this as desired.
The top image shows a gating task with a 365 resolution for the plots. The bottom image shows a gating task after changing the plot resolution to 200 for all selected plots.
4.5.2 Bulk Swap X and Y axis for the main plots
Select the plots you want to swap the x and y axis for.
In the Plot Settings, click on Swap X and Y axis selection.
The image above shows the main plots x and y axis has been swapped. Note that the swap will not occur for any open subplots.
You can also change the X/Y Display Bounds, the Y-Axis selection, and the X-Axis selection for the selected plots in the Plot Settings.
4.6.1 Removing One Plot from View
Hover over the plot you would want to remove. Click the remove button (X) on the plot you would want to remove.
4.6.2 Remove Selected Plots
Select the plots you want to remove from view. See Step 4.4 for this.
Right click on a plot and select Remove selected plot(s).
The plot that you right click on does not have to be a selected plot. You can right click on any plot to bring up the plot menu.
4.6.3 Remove All Plots
Right click on a plot and select Remove all plot(s). This will remove all plots from view and retain the left most plot.
Useful Tip: Collapsing/Opening the Gating Tree View
Click on the collapse gating tree button to collapse the gating tree.
Click on the open gating tree button to open the gating tree.
