Calculate the Geometric Mean Fluorescent Intensity by using the Export Statistics Task.
1. Add a Export Statistics task to your workflow
Click Add new child task and select Export Stats from the task selector. Note that your Workflow might look slightly different, if you are using Compact View.
You can also access the Export Statistics task from within other Have a look below the Logout button (see example in the image below).
2. Setup your Export Statistics task
2.1. Select your Files
Select the Files you want to export MFI for and click Next or click Features. Note that the something or rather about filter selection.
3. Select your Features
Select the Filters you want to export Geometric MFI for → select the Geometric Mean Fluorescent Intensity in Feature Measures → select the Features you want the MFI for. Note that you can select multiple other Measures. You can either click Next to add more data to your Export Statistics task, or click Configuration if you only wish to export the data shown in this step.
In this example, we will export the Geometric Mean Fluorescent Intensities for CCR5, CCR7 and CCR6 on CD4+, CD8+, CD4+CD8+ and DN cells.
In OMIQ we calculate the true Geometric Mean, this means that it is only defined for positive values. Therefore, Filters that include ONLY positive values, will return a result. In other words, if there is a single cell with a value of ≤ 0, within the selected Filter then this will return a NaN result.
4. Configure your Export settings
Select the File Format you want to export to, either as a CSV file or a GraphPad Prism file. Adjust any other settings and click Export or Run Export Stats.
Change the Exported Data Units to Scaled per Scaling Settings if you prefer to report any scaling you have applied in workflow.
5. Download your File
Navigate the the Results tab within the Export Statistics task and click the cloud icon to download your file.